Journal: Frontiers in Oncology

Article Title: Establishment, characterization, and drug screening of low-passage patient individual non-small cell lung cancer in vitro models including the rare pleomorphic subentity

doi: 10.3389/fonc.2023.1089681

Figure Lengend Snippet: Antibodies used for flow cytometry.

Article Snippet: LYPD3 , APC , Sino Biological Europe GmbH, Düsseldorf, Germany , 11836-H08H.

Techniques: Cytometry

Journal: Frontiers in Oncology

Article Title: Establishment, characterization, and drug screening of low-passage patient individual non-small cell lung cancer in vitro models including the rare pleomorphic subentity

doi: 10.3389/fonc.2023.1089681

Figure Lengend Snippet: Flow cytometry. Histogram overlays of unstained controls (dotted lines for all three cell lines) and measurements for HROLu55 (green), HROLu22 (blue), and HROBML01 (red) for the epitopes CD326, PD-L1, EGFR, CD26, LYPD3, DSG3, CCD59, CD27, and CD90 are shown.

Article Snippet: LYPD3 , APC , Sino Biological Europe GmbH, Düsseldorf, Germany , 11836-H08H.

Techniques: Flow Cytometry

Journal: Molecular Human Reproduction

Article Title: Differential tissue-specific damage caused by bacterial epididymo-orchitis in the mouse

doi: 10.1093/molehr/gaaa011

Figure Lengend Snippet: RT-qPCR analysis and comparison of modulation of antimicrobial factors in caput and cauda epididymidis and Lypd8 assessment in mouse and in vitro assay. ( A ) beta-defensins, ( B ) UPEC-associated antimicrobial factors. Unpaired t test ( * ) or Mann–Whitney U test (#) for each organ, respectively, comparing sham to UPEC-infected mice ( **** P < 0.0001, *** P < 0.001, ** P < 0.01, * P < 0.05). Fold change calculated via 2 (−ddCT) method. Data presented as box-and-whisker with median and min/max. cap = caput, cau = cauda. Sham 10 days, caput n = 4, cauda n = 4; UPEC 10 days, caput n = 4, cauda n = 6. ( C ) Laser-assisted microdissection of caput epithelium and caput interstitium from wild-type epididymis, n = 2. Relative expression calculated via 2 (−dCT) method. Data presented as mean ± SD. ( D ) Bacterial adherence to MEPC5 cells under the presence (+) or absence (−) of 1 μg/ml human recombinant LYPD8 (hLYPD8). CFU/ml were calculated after incubation of agar plates at 37°C for 24 h. Summary of three independent experiments with each time n = 2–3 per group. Unpaired t test comparing the number of MEPC5-adherent UPEC in the presence or absence of LYPD8 ( **** P < 0.0001, *** P < 0.001, ** P < 0.01, * P < 0.05).

Article Snippet: To assess the effects of LYPD8 on bacterial adherence to epididymal epithelial cells in vitro , UPEC were pre-incubated for 1 h on ice with or without 1 μg/ml human recombinant LYPD8 (product no. 9087-C4, Novus Biological, CO, USA) ( Okumura et al ., 2016 ) before infection of MEPC5 cells.

Techniques: Quantitative RT-PCR, Comparison, In Vitro, MANN-WHITNEY, Infection, Whisker Assay, Laser Capture Microdissection, Expressing, Recombinant, Incubation

Journal: The FEBS journal

Article Title: Stimulation of Pol III-dependent 5S rRNA and U6 snRNA gene expression by AP-1 transcription factors.

doi: 10.1111/febs.14104

Figure Lengend Snippet: Fig. 1: In silico prediction and validation of putative AP-1 binding sites in Pol III-target gene

Article Snippet: All rights reserved. subunit of RNA polymerase III (POLR3D) were obtained from Proteintech (Rosemont, IL, USA).

Techniques: In Silico, Biomarker Discovery, Binding Assay

Journal: The FEBS journal

Article Title: Stimulation of Pol III-dependent 5S rRNA and U6 snRNA gene expression by AP-1 transcription factors.

doi: 10.1111/febs.14104

Figure Lengend Snippet: Fig. 5: Recruitment of AP-1 transcription factors to Pol III-transcribed gene promoters. (A)

Article Snippet: All rights reserved. subunit of RNA polymerase III (POLR3D) were obtained from Proteintech (Rosemont, IL, USA).

Techniques:

Journal: The FEBS journal

Article Title: Stimulation of Pol III-dependent 5S rRNA and U6 snRNA gene expression by AP-1 transcription factors.

doi: 10.1111/febs.14104

Figure Lengend Snippet: Fig. 6: Epigenetic changes at Pol III-transcribed gene promoters mediated by AP-1 factors.

Article Snippet: All rights reserved. subunit of RNA polymerase III (POLR3D) were obtained from Proteintech (Rosemont, IL, USA).

Techniques:

Journal: Molecular Neurobiology

Article Title: Novel Exosome Biomarker Candidates for Alzheimer’s Disease Unravelled Through Mass Spectrometry Analysis

doi: 10.1007/s12035-022-02762-1

Figure Lengend Snippet: AACT exosomal levels in dementia and AD cases monitored by distinct antibody-based approaches. AACT levels were assessed through immunoblot analysis or commercial ELISA assays in serum-derived exosomes from Controls (CDR = 0 and MMSE −) and individuals with dementia (CDR ≥ 1 and MMSE +) from UA-cohort ( a , d ), and AD clinically diagnosed cases from UA-cohort ( b , e ) or UMG-cohort ( c , f ). For WB, each point represents the relative densitometry ratio. For ELISA, each point represents the mean concentration value obtained for each individual. The solid horizontal line shows mean, and error bars indicates standard deviations. Abbreviations: AD, Alzheimer’s disease; C, Controls; CDR, Clinical Dementia Rate; MMSE, Mini-Mental State Examination. * p ≤ 0.05

Article Snippet: Further, AACT and C4BPα levels were also evaluated by enzyme-linked immunosorbent assay (ELISA), in serum-derived exosomes of the same individuals, using the commercial Human AACT ELISA Kit (ab217779; Abcam) or the Human C4 binding protein A ELISA Kit (NBP2-60,550; Novus Biologicals), according to manufacturer’s instructions.

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Derivative Assay, Concentration Assay

Journal: Proteomics. Clinical applications

Article Title: Use of a Targeted Urine Proteome Assay (TUPA) to Identify Protein Biomarkers of Delayed Recovery After Kidney Transplant

doi: 10.1002/prca.201600132

Figure Lengend Snippet: Relative Expression of 107 Urine Proteins in Kidney Transplant Patients as Determined by TUPA MRM Analyses 1

Article Snippet: ELISA Assays C4b-binding Protein Alpha Chain (C4BPA) was quantified with sandwich ELISA kit EC2202-1 from Assaypro.

Techniques: Expressing

Journal: Proteomics. Clinical applications

Article Title: Use of a Targeted Urine Proteome Assay (TUPA) to Identify Protein Biomarkers of Delayed Recovery After Kidney Transplant

doi: 10.1002/prca.201600132

Figure Lengend Snippet: Trajectory of Fold-Changes for the Top 4 Proteins. The fold-changes for the top 4 proteins, C4b-binding protein alpha chain (C4BPA), Serum amyloid P-component (SAMP), Immunoglobulin superfamily member 8 (IGSF8), and Guanylin (GUC2A) were determined by TUPA analyses described in the text and summarized in Table 1.

Article Snippet: ELISA Assays C4b-binding Protein Alpha Chain (C4BPA) was quantified with sandwich ELISA kit EC2202-1 from Assaypro.

Techniques: Binding Assay

Journal: Proteomics. Clinical applications

Article Title: Use of a Targeted Urine Proteome Assay (TUPA) to Identify Protein Biomarkers of Delayed Recovery After Kidney Transplant

doi: 10.1002/prca.201600132

Figure Lengend Snippet: Average ELISA and MRM Fold-Changes for C4b-binding Protein Alpha Chain and Serum Amyloid P-Component

Article Snippet: ELISA Assays C4b-binding Protein Alpha Chain (C4BPA) was quantified with sandwich ELISA kit EC2202-1 from Assaypro.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: Toxoplasma gondii Recruits Factor H and C4b-Binding Protein to Mediate Resistance to Serum Killing and Promote Parasite Persistence in vivo

doi: 10.3389/fimmu.2019.03105

Figure Lengend Snippet: Toxoplasma gondii recruits AP regulator Factor H and CP/LP regulator C4b-binding protein to the parasite surface. 1 × 10 6 Type II ME49 parasites were incubated in 10% NHS for 0–60 min at 37°C. Western blots (left panels) of C4BP (A) (rabbit α-human C4BP, AssayPro 1:500) and FH (B) (goat α-human Factor H, CompTech 1:20,000) binding. Serum or purified protein was used as a positive control and heat inactivated serum (hiNHS) was used as a negative control. Blots were stripped and re-probed with anti-SRS29B (SAG1) for loading control. Images are from one representative of three independent experiments with similar results. Right panels (A,B) represent flow cytometric assays of C4BP (A) and FH (B) binding to the parasite surface for 0–60 min. Heat inactivated NHS (hiNHS) serum was used as a negative control. Flow cytometry data are shown as mean ± SEM from three independently performed experiments.

Article Snippet: The following antibody dilutions were used: rabbit anit-SRS29B (formerly SAG1, 30 kDa) 1:5,000, rabbit anti-C4BP (alpha chain, 70 kDa) (AssayPro) 1:1,000, goat anti-C3 (C3, C3b, iC3b) and goat anti-Factor H (full length protein, 155 kDa) antibodies (CompTech) were both used at 1:20,0000, anti-rabbit HRP 1:10,000 (Sigma), and anti-goat HRP 1:5,000 (Santa Cruz Biotechnology, Inc.).

Techniques: Binding Assay, Incubation, Western Blot, Purification, Positive Control, Negative Control, Flow Cytometry

Journal: Frontiers in Immunology

Article Title: Toxoplasma gondii Recruits Factor H and C4b-Binding Protein to Mediate Resistance to Serum Killing and Promote Parasite Persistence in vivo

doi: 10.3389/fimmu.2019.03105

Figure Lengend Snippet: Factor H and C4b-binding protein contribute to serum resistance. Factor H (FH) and C4b-binding protein (C4BP) were blocked by pre-incubating 10% NHS with 1:100 or 1:400 dilution of goat α-human FH (CompTech) or rabbit α-human C4BP (AssayPro) for 1 h on ice before adding to 1 × 10 6 parasites and incubating for 60 min at 37°C. Flow cytometric analysis of C5b-9 formation (A) and parasite viability (B) after 60' in 10% NHS blocked with 1:100 or 1:400 of α-C4BP (gray bars) α-FH (open bars) antibodies. 10% heat inactivated serum (hiNHS) was used a negative control. Flow cytometry data are shown as mean ± SEM from three independently performed experiments. Significant differences between the compared groups was determined using multiple Student's t test with Holm-Sidak correction for multiple comparisons, * p < 0.05, *** p < 0.001, **** p < 0.0001.

Article Snippet: The following antibody dilutions were used: rabbit anit-SRS29B (formerly SAG1, 30 kDa) 1:5,000, rabbit anti-C4BP (alpha chain, 70 kDa) (AssayPro) 1:1,000, goat anti-C3 (C3, C3b, iC3b) and goat anti-Factor H (full length protein, 155 kDa) antibodies (CompTech) were both used at 1:20,0000, anti-rabbit HRP 1:10,000 (Sigma), and anti-goat HRP 1:5,000 (Santa Cruz Biotechnology, Inc.).

Techniques: Binding Assay, Negative Control, Flow Cytometry